Reagent Selection for Multicolor Flow Cytometry
Multicolor flow cytometry allows simultaneous analysis of multiple cellular markers. Reagent selection plays a crucial role in successful multicolor experiments.
Fluorochrome compatibility and spectral overlap must be carefully managed. Poor panel design can lead to signal interference and inaccurate interpretation.
Antibody titration ensures optimal staining intensity. Using excessive reagent can increase background noise, while insufficient amounts reduce sensitivity.
Controls such as isotype and compensation controls support accurate data analysis.
Thoughtful reagent selection enhances experiment efficiency and data reliability in complex analyses.
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